Fig. 8

Analysis of the distribution of telomeres by performing FISH using a telomere probe in combination with immunocytochemical labeling of Sycp3 in spermatocytes of the imo zebrafish mutant. FISH and labeling of Sycp3 were performed in the imo spermatocytes at leptotene (A), early zygotene (B), and zygotene (C) stages. A: Telomeres localize within a small region of the nuclear periphery and form a bouquet configuration at the leptotene stage. B: At the early zygotene stage, telomeres start to move over the nuclear periphery. C: Telomeres disperse over the nuclear periphery at the zygotene stage. Left panels show Sycp3 staining (green), middle panels show FISH signals (magenta), and right panels show merged images of the left and middle panels. Nuclei were stained with TO-PRO-3 (blue). Scale bar = 10 µm. These images are representative of 20 nuclei analyzed at each stage per specimen (n = 3).