FIGURE

Fig. 3

ID

ZDB-FIG-150311-9

Publication

Le Pabic et al., 2014 - Fat-Dachsous Signaling Coordinates Cartilage Differentiation and Polarity during Craniofacial Development

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Fig. 3

fat3, dchs2 and fjx1 are expressed in cartilage precursors.

(A–F): In situ hybridization for fat3, dchs2 and fjx1. Lateral views, anterior to the left. fat3 (blue/black) is coexpressed with col2a1 (red) in presumptive cartilages at 54 hpf (A) and 60 hpf (B). dchs2 (C, D) and fjx1 (E, F) are also expressed in presumptive pharyngeal cartilages at 54 and 60 hpf. Scale bar = 54 µm. (G–H′): Fat3 protein localizes to the cell membrane but is more diffuse in prechondrocytes than in surrounding cells. (G–H): Two-channel images showing sox10:lyn-tdTomato membrane labeling (red) and anti-Fat3 signal (green). (G′-H′): Single-channel images showing anti-Fat3 signal alone. Scale bar = 21µm. Mc: Meckel′s; pq: palatoquadrate.

Expression Data

Genes:	col2a1a dchs2 fat3a fjx1 Tomato
Fish:	WT ir1040Tg (AB)
Anatomical Terms:	Meckel's cartilage palatoquadrate cartilage
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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