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Neither zebrafish ncor2 nor ncor1 is required for expressions of early transcriptional factors tal1, lmo2, and etv2. A–I,A2–I2: Whole-mount in situ hybridization was performed on the embryos (myoD: a marker to indicate the developmental stage) to examine the expressions of tal1 (A–C,A′–C′), lmo2 (D–F,D′–F′) and etv2 (G–I,G′–I′). Embryos at the one- to two-cell stage were microinjected with control MO (A,A′,D,D′,G,G′), ncor2 MO (B,B′,E,E′,H,H′) and ncor1 MO (C,C′,F,F′,I,I′) and then grown to 10-somite stage, respectively. Embryos were positioned anterior top and viewed laterally (A–I) or anterior left and viewed dorsally (A′–I′). J: Quantitative real-time PCR was performed to confirm the relative expression level changes of tal1, lmo2, and etv2 in ncor2 or ncor1 morphants. Experiments were repeated three times. Results were subjected to Student′s t-test.
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