FIGURE

Fig. 4

ID

ZDB-FIG-150311-23

Publication

Hu et al., 2014 - Katanin p80 Regulates Human Cortical Development by Limiting Centriole and Cilia Number

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Fig. 4

Maternal Contribution of katnb1 Is Essential for Zebrafish Gastrulation

(A) Katanin p80 protein and gene structure is highly conserved between humans and zebrafish.

(B) TALENs targeting the genomic region surrounding the zebrafish katnb1 exon 6-intron 6 boundary create a series of mutant alleles. Reference sequence (top). Blue box, TALEN recognition sites; dash, deleted base pairs; red letters, inserted base pairs.

(C) Mutant alleles are predicted to alter protein structure by deletion of amino acids or early truncation of katanin p80 protein.

(D) Progeny of mutant females show a wide spectrum of phenotypes, ranging from early embryonic lethality and anencephaly to microcephaly.

(E) Maternal effect of katnb1 is observed in crosses of mutant females, compared with progeny arising from heterozygous carriers. Embryos developed normally until 70% epiboly.

See also Table S2 and Figure S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	katnb1^mh103/mh103 katnb1^mh101/mh101; katnb1^mh102/mh102
Observed In:	brain gastrulation
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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