FIGURE

Fig. 2

ID

ZDB-FIG-150127-11

Publication

Hüsken et al., 2014 - Tcf7l2 Is Required for Left-Right Asymmetric Differentiation of Habenular Neurons

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Fig. 2

tcf7l2 Mutants Show LR Symmetric Patterns of Epithalamic Neuron Differentiation

(A–F′) Dorsal views, with anterior to the top, of the habenulae of embryos labeled with markers at stages shown to the left. In (D–E′), nuclei (gray) are labeled with Topro3 and Sytox orange, respectively. All markers are symmetric in tcf7l2 mutants. Scale bars in (A)–(C′) represent 25 µm.

(G and G′) Dorsal views of 3D reconstructed confocal images of lipophilic-dye-labeled left (red) and right (green) habenular axons terminating in the IPN. The asterisk highlights the lack of axon terminals normally innervating the ventral IPN.

dHbl and dHbm, lateral and medial habenular subnucleus, respectively; dIPN and vIPN, dorsal and ventral interpeduncular nucleus, respectively; vHb, ventral habenular subnucleus. See also Figures S2 and S3 and Table S1.

Expression Data

Genes:	cadps2 cntn2 EGFP GFP kctd12.2
Antibody:	Ab-SV2
Fish:	WT tcf7l2^u754/u754 tcf7l2^u763/u763 pku588Et rw0110bTg tcf7l2^u754/u754; pku588Et tcf7l2^u754/u754; rw0110bTg tcf7l2^u763/u763; pku588Et tcf7l2^u763/u763; rw0110bTg
Anatomical Terms:	dorsal habenular nucleus habenula ventral habenular nucleus
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tcf7l2^u754/u754 tcf7l2^u763/u763
Observed In:	habenula neuron projection
Stage:	Day 4

Phenotype Detail

Acknowledgments

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