Fig. 2

Rbm24a and Rbm24b are required for normal somitogenesis.

Bright field 8 somite embryos are shown in dorsal orientation (A-C). Spatial expression of myod in somites of 13 somite embryos was detected using anti-sense riboprobes (D-F). Measurements of individual chevron angle measurements of somites 6-10 of 13 somite embryos for uninjected, ctrlMO injected rbm24aMO and rbm24bMO embryos, n = 3-5 embryos each (G). Statistically significant differences in somite angle as compared to ctrlMO is shown using an asterisk. The trunks of 24 hpf embryos are shown in the lateral orientation stained via myod ISH (H-J). Average somite counts of uninjected, ctrlMO injected rbm24aMO and rbm24bMO 13 somite and 24 hpf embryos are shown (K). Number of embryos counted shown on bars. Error bars are standard error of the mean. Significance determined at 95% confidence with significance compared to ctrlMO shown as * = p≤0.05; ** = p≤0.01; *** = p≤0.001 and significance between respective MO only and MO rescue conditions shown as + = p≤0.05; ++ = p≤0.01; +++ = p≤0.001. Trunks of 24 hpf uninjected, ctrlMO injected rbm24aMO, rbm24bMO, rbm24aMO embryos with rbm24a mRNA and rbm24bMO embryos with rbm24b mRNA embryos are shown in the lateral orientation imaged in bright field (L-P), double immunohistochemistry for phalloidin and anti-pFAK (Q-U), phalloidin alone (V-Z).