FIGURE

Fig. 4

ID

ZDB-FIG-141124-12

Publication

Tsuji et al., 2014 - Whole Organism High Content Screening Identifies Stimulators of Pancreatic Beta-Cell Proliferation

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Fig. 4

RA and Prednisolone but not Trazodone, enhance beta-cell regeneration.

(A) Schematic diagram for assessment of beta-cell regeneration. To examine beta-cell regeneration, we made use of the NTR/MTZ beta-cell ablation model. At 80 hpf, after ablating the beta-cells with MTZ from 50–80 hpf, Tg(ins:H2BGFP);Tg(ins:CFP-NTR) larvae were treated with the compounds for 48 h. The numbers of Tg(ins:H2BGFP)⁺ cells were counted at 128 hpf. (B) Confocal images of Tg(ins:H2BGFP)⁺ beta-cells in larvae treated with 1 μM retinoic acid, 10 μM trazodone, or 10 μM prednisolone in 1% DMSO at 128 hpf. Each image is a lateral view, anterior to the bottom and dorsal to the right. (C) Quantification of beta-cell regeneration per larva at 128 hpf, following treatment with hit compounds from 80–128 hpf. Error bars represent SEM. *P<0.05 compared to DMSO treated controls; n = 13–16 larvae for each group.

Expression Data

Gene:	Kaede
Fish:	s892Tg; s949Tg
Conditions:	chemical ablation: insulin secreting cell, chemical treatment: metronidazole chemical ablation: insulin secreting cell, chemical treatment: prednisolone chemical ablation: insulin secreting cell, chemical treatment: all-trans-retinoic acid chemical ablation: insulin secreting cell, chemical treatment: metronidazole
Anatomical Term:	pancreatic B cell
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s892Tg; s949Tg
Conditions:	chemical ablation: insulin secreting cell, chemical treatment: prednisolone chemical ablation: insulin secreting cell, chemical treatment: all-trans-retinoic acid
Observed In:	pancreatic B cell regeneration
Stage:	Day 5

Phenotype Detail

Acknowledgments

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