Fig. 4
- ID
- ZDB-FIG-141016-43
- Publication
- Hudish et al., 2013 - miR-219 Regulates Neural Precursor Differentiation by Direct Inhibition of Apical Par Polarity Proteins
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miR-219 Has Single, Conserved Target Sites within prkci and pard3 32 UTRs (A and B) Schematic representations of prkci and pard3 transcripts with predicted miR-219 target sites conserved among various species. (C–E) In controls at 1 and 2 dpf, Prkci protein is concentrated at apical membranes lining the primitive lumen, but by 3 dpf Prkci is limited to the central canal (brackets). (F and G) Prkci labeling persists along a primitive lumen extending across the spinal cord dorsoventral axis in 3 dpf and 5 dpf miR-219 MO-injected larvae. (H) Sequences (220 bp) from the pard3 32 UTR containing wild-type and mutated miR-219 target sites were cloned into dual luciferase vectors. (I) Quantification of light units revealed a miR-219-mediated reduction of reporter gene expression that was abrogated by 1 and 2 bp mutations within the target site. Data represent ± SEM (three independent experiments). Brackets indicate pairwise comparisons. p < 0.0001, unpaired t test. Scale bar equals 10 μm. |
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| Stage Range: | Prim-5 to Day 5 |
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| Stage Range: | Protruding-mouth to Day 5 |
Reprinted from Developmental Cell, 27(4), Hudish, L.I., Blasky, A.J., and Appel, B., miR-219 Regulates Neural Precursor Differentiation by Direct Inhibition of Apical Par Polarity Proteins, 387-398, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell