FIGURE

Fig. 5

ID

ZDB-FIG-141001-8

Publication

Shi et al., 2014 - Acetylcholine serves as a derepressor in Loperamide-induced Opioid-Induced Bowel Dysfunction (OIBD) in zebrafish

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Fig. 5

(a) Immunohistochemical staining of HuC/D in both control and LH-treated fish at 6 dpf. The data show no significant difference between the control and chemical application groups. Left panels show the whole body staining pattern of HuC/D, whereas middle and right panels represent high magnifications of the areas that are boxed in left. Right panels are the images of middle merged with DIC. (b) The AChE activity detection assay reveal that AChE activity is significantly reduced after LH treatment (right panels) compared with control (left panels) in the gut but not elsewhere in the body. Upper panels show the whole body staining pattern of Ache, whereas bottom panels represent high magnifications of the boxed images. The red arrows in b indicate the intestinal bulb region where peristalsis is identified.

Expression Data

Antibody:	Ab1-elavl
Fish:	WT
Condition:	chemical treatment: loperamide hydrochloride
Anatomical Term:	enteric neuron
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: loperamide hydrochloride
Observed In:	gut acetylcholinesterase activity
Stage:	Day 6

Phenotype Detail

Acknowledgments

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