FIGURE

Fig. 6

ID

ZDB-FIG-140908-6

Publication

Benato et al., 2014 - A living biosensor model to dynamically trace glucocorticoid transcriptional activity during development and adult life in zebrafish

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Fig. 6

Reduced GRE activity following treatment with the GR antagonist RU486. Two-dpf transgenic embryos were treated for 24 h with 10 µM DEX alone or combined with three different RU486 concentrations (50, 250 and 1250 nM). Left panel: fold changes in gene expression of fkbp5 and egfp in treated embryos as compared to only DEX-treated control (set at 1). The expression levels of the target genes were normalized on ef1a as housekeeping gene. Values represent the mean ± S.E. Asterisks indicate expression levels that are significantly different from control (DEX-treated ia20): *P < 0.05; **P < 0.01; ***P < 0.001. Right panel: WMISH of egfp mRNA after DEX treatment alone or combined with RU486 at different concentrations. Scale bar: 200 µM.

Expression Data

Gene:	EGFP
Fish:	ia20Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 392(1-2), Benato, F., Colletti, E., Skobo, T., Moro, E., Colombo, L., Argenton, F., Dalla Valle, L., A living biosensor model to dynamically trace glucocorticoid transcriptional activity during development and adult life in zebrafish, 60-72, Copyright (2014) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.