FIGURE

Fig. 3

ID

ZDB-FIG-140903-49

Publication

Carnes et al., 2014 - Discovery and Functional Annotation of SIX6 Variants in Primary Open-Angle Glaucoma

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Functional evaluation of SIX6 variants on the volume of the optic nerve.

Representative whole mount images of acetylated-tubulin expression in the heads of zebrafish embryos injected with a control or six6a morpholino, rescued by co-injection with human non-risk SIX6 transcript or a transcript containing the Leu205Arg hypomorphic variant (A). Acetylated-tubulin staining is restricted primarily to axon tracts and can be used to visualize the optic nerve. Relative to the control morphants, volumetric regions of interest (ROI) along the optic nerve in six6a morphants were reduced significantly. Co-injection of human variants revealed a hypomorphic (Leu205Arg, Asn141His) or benign (Glu93Gln) role of the variants on the optic nerve (B). Sample size for all injection paradigms ranged from 7–9 and p-values are plotted for each comparison (*** p<0.001; ** p<0.01). No significant changes in the volume of other axonal tracts in the head (marked by an asterisk) were detected. Standard error of the mean is shown and white scale bars = 20 μm.

Expression Data

Antibody:	Ab1-tuba
Fish:	WT WT + MO1-six6a
Condition:	chemical treatment: messenger RNA
Knockdown Reagent:	MO1-six6a
Anatomical Term:	cranial nerve II
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-six6a
Condition:	chemical treatment: messenger RNA
Knockdown Reagent:	MO1-six6a
Observed In:	cranial nerve II optic nerve development
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.