FIGURE

Fig. S1

ID

ZDB-FIG-140811-25

Publication

Zaghloul et al., 2010 - Functional analyses of variants reveal a significant role for dominant negative and common alleles in oligogenic Bardet-Biedl syndrome

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Fig. S1

Suppression of bbs genes in vivo produces early gastrulation phenotypes. (A) Injection of splice-blocking (SB) MO against bbs1 or bbs6 results in complete suppression of message as shown by PCR amplification of cDNA reverse transcribed from extracted total mRNA. β-actin was used as a control. (B) SB MO produces similar phenotypes to those produced by translation-blocking MO and in similar proportions (C). Consistent with these similar defects, co-injection of SB MO with mutant mRNA for a subset of bbs1 or bbs6 mutations produced defects similar to those produced by coinjection with TB MO. (D-F) To verify specificity of each BBS mRNA to rescue the corresponding bbs MO, we attempted to rescue bbs1, bbs7, bbs4, bbs6, or bbs3 with either bbs7, bbs1, bbs6, or bbs4, respectively, and found that the mismatched mRNA did not rescue the morphant phenotypes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-bbs1 WT + MO4-mkks
Knockdown Reagents:	MO4-bbs1 MO4-mkks
Observed In:	gastrulation notochord somite whole organism anterior-posterior axis
Stage Range:	5-9 somites to 10-13 somites

Phenotype Detail

Acknowledgments

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