FIGURE

Fig. 4

ID

ZDB-FIG-140715-13

Publication

Petrie et al., 2014 - Macrophages modulate adult zebrafish tail fin regeneration

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Fig. 4

Macrophages modulate the proliferative capacity of the regeneration blastema. (A) Hematoxylin-stained sections of tail fin regenerates (blastemal region) at 3 dpa. Macrophage-depleted fins (right) display slightly reduced numbers of deep mesenchymal cells of the blastema. Arrowheads indicate the plane of amputation. (B) Blastemal and macrophage proliferation assessed by staining 2 (iii,iv) or 3 (i,ii) dpa regenerates for PCNA (i-iv) or L-plastin (i,ii), a marker for leukocytes (mostly macrophages), and with DAPI. Scale bars: 20µm. (C) Quantification of the length of the blastema in macrophage-depleted (NTR+MTZ; n=7) and wild-type (n=6) fins at 3dpa. Macrophage-depleted fins displayed slightly decreased blastemal size compared with wild-type fins. (D) Cell proliferation (PCNA⁺ cells) quantified in the blastema is reduced in NTR+MTZ compared with wild-type controls. PCNA⁺ cell number was averaged among all sections spanning the entire fin width, and normalized to DAPI counts in the image. WT+MTZ, n=10; NTRMTZ, n=8; NTR+MTZ, n=9. *P=0.0425 (two-tailed t-test, error bars indicate s.e.m.).

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

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