FIGURE

Fig. 1

ID

ZDB-FIG-140527-8

Publication

Steiner et al., 2014 - Dynamic gene expression by putative hair-cell progenitors during regeneration in the zebrafish lateral line

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Fig. 1

Expression of fluorescent proteins in mantle cells of the posterior lateral-line system. (A) Confocal mosaic images of a living alpl:mCherry;Et20 larva at 4 days postfertilization (dpf) demonstrate expression of mCherry (magenta) overlapping with that of GFP (green) in neuromasts and interneuromast cells. (Scale bar: 500 μm.) The same color code applies in C–E. (B) A neuromast comprises at least three cell types: hair cells (green), supporting cells (aqua), and mantle cells (magenta). (C) mCherry expression in alpl:mCherry larvae is limited to a subset of cells at the periphery of the neuromast. The image represents a confocal slice through a living alpl:mCherry; Tg(8.0cldnb:lynEGFP)zf106 larva, in which all cells of the neuromast express membrane-tethered GFP. (D) An alpl:mCherry;pou4f3:GFP animal expresses mCherry in peripheral cells, but that marker is excluded from hair cells that express membrane-tethered GFP instead. (E) mCherry and GFP have extensively overlapping but not identical expression patterns in mantle cells of alpl:mCherry;Et20 larvae. The arrowheads indicate two mCh⁺, GFP cells. (Scale bar: C–E, 10 μm.)

Expression Data

Genes:	EGFP GFP mCherry
Fish:	ru1011Tg; s356tTg ru1011Tg; sqet20Et ru1011Tg; zf106Tg
Anatomical Terms:	fin interneuromast cell neuromast plasma membrane neuromast hair cell neuromast mantle cell pigment cell posterior lateral line neuromast
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA