Fig. S5
- ID
- ZDB-FIG-140523-50
- Publication
- Swift et al., 2014 - SoxF factors and Notch regulate nr2f2 gene expression during venous differentiation in zebrafish
- Other Figures
- All Figure Page
- Back to All Figure Page
The Tp1:mCherry and efnb2a:eGFP transgenic reporters are responsive to reduced Notch signaling. (A,B) Whole mount in situ hybridization of 24 hpf zebrafish probed for dll4. (C,D) Red fluorescent confocal micrographs of 24 hpf Tg(Tp1bglob:hmgb1- mCherry)h11 (“tp1:mCherry”) transgenic zebrafish. (E,F) Green fluorescent confocal micrographs of 36 hpf Tg(efnb2a:egfp) transgenic zebrafish. Animals were either treated with control DMSO carrier (A,C,E) or with 100µm Notch inhibitor DAPT (B,D,F). Animals in DMSO/DAPT panel pairs were imaged using identical conditions for comparison. Superficial image planes were removed for visualization of vessels in the reconstructions shown in panels E and F because of extensive fluorescence in muscle and epidermis in this transgenic line. (G) Quantitative RT-PCR measurement of dll4 and grl gene expression in excised trunks (see Materials and Methods) of 24 hpf animals. Values are all normalized to control gene expression levels, which are set equal to 1. Rostral is to the left and dorsal is up in all image panels. Scale bars, (A-D) 200 µm, (E,F) 50 µm. |
Reprinted from Developmental Biology, 390, Swift, M.R., Pham, V.N., Castranova, D., Bell, K., Poole, R.J., Weinstein, B.M., SoxF factors and Notch regulate nr2f2 gene expression during venous differentiation in zebrafish, 116-25, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.