Fig. 6

The effect of smyd1a knockdown on the sarcomere organization in fast muscles.

Zebrafish embryos co-injected with smyd1a E8I8 and E9I9 MOs were fixed at 28, 48 and 72 hpf. The sarcomere organization of thin filaments and Z-lines was analyzed by immunostaining with anti-α-actin and anti-α-actinin antibody, respectively, and followed by FTIC-labeled secondary antibody. The images represent side view of trunk muscles around segment 10. A–C. Lateral view of thin filament organization in skeletal muscle fibers of control-MO injected embryos at 28 (A), 48 (B) and 72 (C) hpf. D–F. Lateral view of thin filament organization in skeletal muscle fibers of smyd1a MO injected embryos at 28 (D), 48 (E) and 72 (F) hpf. G–I. Lateral view of Z-line organization in skeletal muscle fibers of control-MO injected embryos at 28 (G), 48 (H) and 72 (I) hpf. J–L. Lateral view of the Z-line organization in skeletal muscle fibers of smyd1a MO injected embryos at 28 (J), 48 (K) and 72 (L) hpf. Scale bars: 20 μm in D–F and J–L.