FIGURE

Fig. 2

ID

ZDB-FIG-140422-64

Publication

West et al., 2014 - Two types of transgenic lines for doxycycline-inducible, cell-specific gene expression in zebrafish ultraviolet cone photoreceptors

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Time course of doxycycline-induced GFP expression in Tg(opn1sw1:rtTA, TRE:GFP); alb^/ larvae. Confocal z-projections of retinal sections from 6 dpf Tg(opn1sw1:rtTA, TRE:GFP); alb^/ larvae that were (A) untreated or treated with 10 μg/mL Dox for (B) 2, (C) 4, (D) 8, (E) 16, (F) 24, (G) 48, or (H) 72 h. (A–H) Anti-UV opsin immunofluorescence (red) labels UV cone outer segments. GFP fluorescence (green) is apparent after 8 h of treatment (D, D inset) and continues to increase by number of cones with the increase in Dox exposure time (E–H). (I) Anti-GFP immunofluorescence (green) in untreated transgenic larvae indicates GFP expression that is not present in untreated, wild type larvae (J). Scale bar (A), 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Gene expression patterns : GEP, 14(2), West, M.C., Campbell, L.J., Willoughby, J.J., and Jensen, A.M., Two types of transgenic lines for doxycycline-inducible, cell-specific gene expression in zebrafish ultraviolet cone photoreceptors, 96-104, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns