FIGURE

Fig. 1

ID

ZDB-FIG-140409-27

Publication

Cruz et al., 2013 - Glucocorticoid receptor, but not mineralocorticoid receptor, mediates cortisol regulation of epidermal ionocyte development and ion transport in zebrafish (danio rerio)

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Fig. 1

Effect of corticosteroid receptor gene knockdown on NaRC number.

Zebrafish embryos at the 1~4 cell-stage were microinjected with glucocorticoid receptor ATG-MO (GR-ATG), GR-splice variant MO (GR-SV), mineralocorticoid receptor ATG-MO (MR-ATG), or Random-MO (RMO; control). Representative images of yolk-sac NaRCs labeled with anti-α sub-unit of Na⁺-K⁺-ATPase (NKA) in RMO (A), GR-ATG (B), GR-SV (C), and MR-ATG (D) morphants. NaRC numbers are compared in (E). Values are presented as the mean ±s.d. (n = 10–12). ^abIndicates statistically significant differences (<0.05) between groups as determined by one-way ANOVA (Tukey’s pair-wise comparison). Scale bar: 100 μm (A–I).

Expression Data

Antibody:	Ab1-atp1a
Fish:	WT WT + MO1-nr3c1 WT + MO2-nr3c1 WT + MO2-nr3c2
Knockdown Reagents:	MO1-nr3c1 MO2-nr3c1 MO2-nr3c2
Anatomical Term:	NaK ionocyte
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-nr3c1 WT + MO2-nr3c1 WT + MO2-nr3c2
Knockdown Reagents:	MO1-nr3c1 MO2-nr3c1 MO2-nr3c2
Observed In:	NaK ionocyte
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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