FIGURE

Fig. 5

ID

ZDB-FIG-140404-7

Publication

Gray et al., 2014 - Loss of col8a1a function during zebrafish embryogenesis results in congenital vertebral malformations

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Fig. 5

Segmentation of the vertebral column and localization of osteoblasts proceeds on a dysmorphic notochord template. Alcian blue stained larvae (14dpf) (A–B′′′) with lateral insets (red dashed box; A′, B′) and ventral insets (green dashed box; A′′′, B′′′). (A–A′′′) WT larvae display well-formed and segmented centra. (B–B′′′) lev^vu41-/- larvae display abberant apposition of centra (red brackets) and hemicentra (red arrow). Confocal projections of zebrafish vertebral column at 21dpf, illustrating boney matrix (alizarin red in magenta) and osteoblasts (Tg(sp7:EGFP)b1212 in green) (C–F′). (C, D) WT vertebrae and associated osteoblasts are well segmented along the vertebral axis. (E, E′) An individual lev^vu41-/- mutant displaying segmented colocalization of osteoblast and centra, including malformed hemivertebrae (yellow arrows) that continues to have mostly well segmented osteoblasts (E′), excepting some ectopic osteoblast-mineral formations in regions previously devoid of osteoblasts or mineral (E′, yellow asterix). (F and F′) An individual lev^vu41-/- mutant displaying regions of severe notochord bending with aberrantly apposed osteoblasts (F, yellow bracket), those progresses to a region with more complete osteoblast coverage (F′, yellow bracket). (C,D) 10dpf; ~SL3-4 (E,F) 10dpf; ~SL3-4 (E′, F′) 14dpf; ~SL4-5. Scale bars for (A, B, A3, B3)=1 mM; (C–F′) and (A′, A′′′,B′,B′′′)=100 mM.

Expression Data

Gene:	EGFP
Fish:	b1212Tg col8a1a^vu41/vu41; b1212Tg
Anatomical Term:	osteoblast
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	col8a1a^vu41/vu41 col8a1a^vu41/vu41; b1212Tg
Observed In:	centrum vertebra vertebral column vertebral column osteoblast
Stage Range:	Days 7-13 to Days 14-20

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 386(1), Gray, R.S., Wilm, T.P., Smith, J., Bagnat, M., Dale, R.M., Topczewski, J., Johnson, S.L., and Solnica-Krezel, L., Loss of col8a1a function during zebrafish embryogenesis results in congenital vertebral malformations, 72-85, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.