FIGURE

Fig. 6

ID

ZDB-FIG-140326-40

Publication

Long et al., 2003 - The zebrafish nodal-related gene southpaw is required for visceral and diencephalic left-right asymmetry

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Fig. 6

The effects of spaw inhibition on midline development are restricted to effects on late lefty1 expression. (A-G) Comparison of lefty1 expression in uninjected (u) and Spaw-MO1-injected (10 ng) embryos at various stages. Dig-lefty1 probe. (A,B) Shield stage. (C,D) One to 3 somite stage. (E,F) Seven to 8 somite stage. (G) nineteen to 22 somite stage. (A,B) View onto blastoderm, dorsal is downwards. (C-F) Lateral views, anterior towards the top. (G) Lateral views, anterior towards the upper right. Arrowheads indicate presence (left) or absence (right) of asymmetric lefty1 expression in diencephalon. Asterisks indicate presence (left) or absence (right) of lefty1 in posterior notochord. (H,I) Comparison of ntl expression in uninjected (u; left in H, right in I) and Spaw-MO1- injected (10 ng; right in H, left in I) embryos at shield and 6-7 somite stages. Dig-ntl probe. (H) Arrowheads indicate ntl expression in dorsal shield and forerunner cells. (I) Views of dorsal midline, anterior is towards top.

Expression Data

Genes:	lft1 tbxta
Fish:	WT WT + MO1-spaw
Knockdown Reagent:	MO1-spaw
Anatomical Terms:	axis dorsal region diencephalon forerunner cell group margin notochord posterior region shield whole organism anterior region whole organism posterior region
Stage Range:	Shield to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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