Fig. 1
Lack of Microglia in nlrc3-like Mutants (A and B) Live 5 dpf zebrafish larvae were stained by neutral red to visualize microglia in the brain. (A) Heterozygous st73/+ larva (arrow points to microglia around optic tectum) is shown. (B) Homozygous st73 larvae have no microglia (arrow). Dorsal views; anterior is to the top. (C and D) Microglial marker apoe expression at 5 dpf in the brain is shown. (E and F) Neutral red-stained heterozygous (E) and homozygous mutant (F) shows normal whole-animal morphologies at 5 dpf. Lateral views; anterior is to the left. (G) Genetic and physical map of the st73 locus. (H) Sequence chromatogram shows the lesion (asterisks) in the coding sequence of nlrc3-like (LOC100538217). (I) The st73 mutation introduces a premature stop codon near the 52 end of the open reading frame of nlrc3-like. See also Figure S1. |
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Stage: | Day 5 |
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Stage: | Day 5 |