FIGURE

Fig. 8

ID

ZDB-FIG-140304-18

Publication

Xu et al., 2014 - Swap70b is required for convergent and extension cell movement during zebrafish gastrulation linking Wnt11 signalling and RhoA effector function

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Fig. 8

Ectopic GFP/Swap70b expression rescued Def6a morphants. Embryos at 2-cell stage were injected with GFP/swap70b mRNA (150 pg) (A), def6a MO (2.5 ng) alone (B), or GFP/swap70b mRNA (150 pg) and def6a MO (2.5 ng) (C) and were stained at tail-bud stage with hgg1 and dlx3b to evaluate CE using the same quantification method described above ( Fig. 4I). (D) Two-tailed Student′s t-tests were used to determine statistical significance. ***: P<0.001. (E) Later at 24 hpf, embryos were grouped into normal (blue), mild (green), moderate (pink) and severe (red) according to their morphological phenotype. Representative images of embryos are shown. (F) Bar graphs show the percentage of embryos with normal, mild, moderate and severe phenotypes. Only 1/189 of the rescued embryos exhibited a severe phenotype as shown in E. n: total number of embryos derived from at least three independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-def6a
Knockdown Reagent:	MO1-def6a
Observed In:	neural plate
Stage:	Bud

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 386(1), Xu, X., Shuen, W.H., Chen, C., Goudevenou, K., Jones, P., and Sablitzky, F., Swap70b is required for convergent and extension cell movement during zebrafish gastrulation linking Wnt11 signalling and RhoA effector function, 191-203, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.