FIGURE

Fig. 7

ID

ZDB-FIG-140212-14

Publication

Palha et al., 2013 - Real-time whole-body visualization of chikungunya virus infection and host interferon response in zebrafish

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Fig. 7

Neutrophils are key ifnφ1-producing cells.

(A-C) Effect of myeloid cell depletion (PU.1 MO, 3 independent experiments), (D-F) of macrophage depletion (metronidazole treatment of mpeg1:Gal4/UAS:NfsB-mCherry fish, 2 independent experiments), (G-I) of neutrophil-biased depletion (csf3r MO, 2 independent experiments), (J-L) of impairment of emergency granulopoiesis (nos2a MO, 2 independent experiments) and (M-O) of hepatocyte depletion (Tomm22 MO, 2 independent experiments) on CHIKV infection. (A, D, G, J and M) Disease score at 3 dpi. (B, E, H, K and N) Survival of infected zebrafish. Data pooled from the independent experiments. (C, F, I, L and O) qRT-PCR of viral E1 transcripts at 24 hpi. Mean ± s.e.m of 6 pools of 5 larvae from 2 (C, F, L) or 1 (I, O) independent experiments. (***P < 0.001; **P < 0.01; *P < 0.05; ns - not significant).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-spi1b WT + MO1-tomm22 WT + MO3-csf3r WT + MO3-nos2a
Condition:	viral treatment: Chikungunya virus
Knockdown Reagents:	MO1-spi1b MO1-tomm22 MO3-csf3r MO3-nos2a
Observed In:	response to virus whole organism
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

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