FIGURE

Fig. 1

ID

ZDB-FIG-140204-35

Publication

Winata et al., 2013 - Genome wide analysis reveals zic3 interaction with distal regulatory elements of stage specific developmental genes in zebrafish

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Fig. 1

Early expression of zic3 as detected by RNA-seq and RT-PCR.

A, UCSC browser image showing RNA-seq reads (pink vertical histograms) at the zic3 locus (in blue; tall boxes - exons; half-height boxes - UTRs; lines - introns; arrowheads - direction of transcription). B, RT-PCR detection of zic3 transcripts in zebrafish embryo. Portion of image showing expression at 1 hpf to 3 hpf was enhanced to show weak band at 3 hpf. C, D and E, zic3 expression in 4 hpf, 6 hpf, and 8 hpf embryos. Lateral view, dorsal to the right. C2, D2 and E2, animal pole view. F, G, zic3 expression in wild-type and gfp in SqET33 embryos at 24 hpf. F2, G2, dorsal view. Note the absence of gfp expression from the olfactory bulb and presomitic mesoderm domains of the wild-type zic3 expression (arrows). H–H3, GFP expression in live SqET33 embryos at 24 hpf. H, dorsal view, H2, frontal view, H3, lateral view.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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