ZFIN Figure: Demir et al., 2013, Fig. 5

Fig. 5

RAB8B Was Required for Wnt/β-Catenin Signaling in Zebrafish and Xenopus

(A) Lateral views of in situ hybridization analysis of Rab8b gene expression between 100% of epiboly and 3 days postfertilization (dpf). Rab8b is expression is enriched in MHB and OV from early somitogenesis through subsequent organogenesis stages (ss, somite stage).

(B) Dorsal views of 30 hr postfertilization (hpf) embryos focused on the head region. Injection of rab8b ATG-morpholino into Tg(7xTCF-XLa.Siam:eGFP)^ia4 transgenic zebrafish expressing GFP upon induction of the synthetic 7XTCF-Siam promoter by β-catenin, resulted in reduced GFP intensity in MHB and OV (n = 49/66), and c-myc expression in the tectum (n = 17/27).

(C) rab8b morpholino 1 (Mo1) and 2 (Mo2) showed similar Wnt loss-of-function phenotypes in Xenopus embryos.

(D and E) Wnt luciferase reporter activity was reduced by rab8b Mos in Xenopus embryos. Embryos were injected with morpholinos and mRNAs as indicated and luciferase reporter assays were performed at gastrula stage. Relative luciferase activity in embryos injected with control-Mo or control-Mo plus mRNA of the indicated activators.

(F) Model.

Error bars represent ±SD of three replicates. See also Figure S5.

Expression Data

Genes:	GFP rab8b
Fish:	WT ia4Tg ia4Tg + MO1-rab8b
Knockdown Reagent:	MO1-rab8b
Anatomical Terms:	brain central nervous system midbrain hindbrain boundary otic placode otic vesicle
Stage Range:	Bud to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.