FIGURE

Fig. 5

ID

ZDB-FIG-140117-21

Publication

Nagashima et al., 2013 - A self-renewing division of zebrafish Muller glial cells generates neuronal progenitors that require N-cadherin to regenerate retinal neurons

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Fig. 5

Induction of neuroepithelial markers in Müller glia is delayed after ouabain. (A) Control (ctrl) HuC/D⁺ neurons. (B) HuC/D immunoreactivity is absent in the GCL and reduced in the INL at 1 dpi. (C) PCNA (green), BLBP (cyan) and Rx1 (magenta) staining at 2 dpi. Right panel: BLBP⁺/Rx1⁺ Müller glia. Scattered PCNA⁺/BLBP^-/Rx1^- cells in the INL and GCL are microglia. (D) At 3 dpi, nGFP⁺/PCNA⁺ (red)/Rx1⁺ (white/magenta) Müller glial nuclei are apically displaced. (E) At 3 dpi, pH3⁺ nuclei (magenta) are apical in the INL but not at the OLM (dotted line). (F) Alcama⁺ (white/magenta) Müller glia radial processes at 5 dpi. (G) At 5 dpi, nGFP⁺/HuC/D⁺ (red) regenerated neurons (higher magnification of boxed area shown in inset). Scale bars: 20 μm (A-G); 2 μm (inset in C).

Expression Data

Genes:	alcama EGFP
Antibodies:	Ab1-elavl Ab1-rx1 Ab3-fabp7 zn-5
Fish:	WT mi2004Tg
Condition:	chemical ablation: retinal neural layer
Anatomical Terms:	amacrine cell Muller cell Muller cell nucleus retina CNS neuron (sensu Vertebrata) retinal ganglion cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT mi2004Tg
Condition:	chemical ablation: retinal neural layer
Observed In:	microglial cell Muller cell Muller cell interkinetic nuclear migration retina retina sensory epithelium regeneration retinal ganglion cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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