FIGURE

Fig. 2

ID

ZDB-FIG-140114-53

Publication

Vo et al., 2013 - Loss of fibrinogen in zebrafish results in symptoms consistent with human hypofibrinogenemia

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Fig. 2

Liver specific expression of fgb-egfp results in incorporation of fusion protein into an induced thrombus.

A fabp10-fgb-egfp transgenic line of zebrafish was produced, and F1 offspring analyzed. (a) Expression was liver specific, as demonstrated by the region of green fluorescence in a 5 dpf larva (yellow signal is yolk sac autofluorescence). (b, c) F1 adult fish displayed overt hemorrhage, suggestive of potential interference with coagulation secondary to dysfibrinogenemia. Pictures of the PCV prior to (d, brightfield+fluorescence; e, fluorescence only), and after (f, brightfield+fluorescence; g, fluorescence only) laser injury (f, g were prior to complete occlusion). The arrowhead in a indicates liver, arrows in d, f indicate PCV and direction of flow, and arrowheads in f, g point to the induced thrombus.

Expression Data

Gene:	EGFP
Fish:	mi4001Tg
Condition:	physical alteration: posterior cardinal vein
Anatomical Terms:	liver posterior cardinal vein
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mi4001Tg
Observed In:	whole organism blood vasculature
Stage:	Adult

Phenotype Detail

Acknowledgments

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