FIGURE

Fig. 3

ID

ZDB-FIG-140107-59

Publication

Weijts et al., 2013 - Atypical E2fs Control Lymphangiogenesis through Transcriptional Regulation of Ccbe1 and Flt4

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Fig. 3

Figure 3. E2f7/8 rescued Ccbe1dependent lymphangiogenesis phenotype.

A, Representative images of Tg(fli1a:gfp;flt1^enh:rfp) un-injected control embryos (nic) or embryos injected with e2f7/8 MOs or mRNA. B, C, D, Quantification of the indicated parameters. Concentrations: e2f7/8 MOs (10 ng each); ccb1 MO (5 ng); e2f7/8 mRNA (100 pg each); ccbe1 mRNA (100 pg).Open arrow heads indicate in (A; upper panel) missing dorsal longitudinal anastomotic vessels. Closed arrow heads indicate (upper panel in A) PLs or (lower panel, A) presence of the TD. All scale bars are 100 μm. Stars indicate missing TD fragments. Data presented as the average (±s.e.m.) compared to the control condition in three independent experiments (*** P<0.001). At least n = 150 embryos per condition in three independent experiments were used for A–D.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hu5333Tg; y1Tg + MO1-ccbe1 hu5333Tg; y1Tg + MO1-ccbe1 + MO1-e2f7 + MO1-e2f8
Knockdown Reagents:	MO1-ccbe1 MO1-e2f7 MO1-e2f8
Observed In:	dorsal longitudinal anastomotic vessel dorsal longitudinal anastomotic vessel sprouting angiogenesis lymphangioblast cord lymphangioblast lymphangiogenesis thoracic duct
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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