FIGURE

Fig. 7

ID

ZDB-FIG-131218-28

Publication

Sittaramane et al., 2013 - The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function

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Fig. 7

Floor plate cilia are largely dispensable for FBM neuron migration. (A–H) Dorsal views of hindbrains with anterior to the left. The ratio of the number of embryos exhibiting a phenotype to the total number of embryos examined is indicated in each panel. (A,B) Anti-tubulin immunostaining (green). In a wildtype hindbrain (A), floor plate cilia (arrow) are uniformly orientated along the anterior-posterior axis, whereas in a vangl2 mutant (tri/) mutant (B), the floor plate cilia (arrow) are disorganized and misorientated. (C,D) Tg(isl1:GFP) embryos processed for anti-tubulin (red) and anti-GFP (green) immunostaining. In a wildtype sibling (C), floor plate cilia (arrow) are uniformly orientated, and FBM neurons (arrowhead) migrate out of r4. In an iguana/ embryo (D), although floor plate cilia are absent, FBM neurons (arrowhead) migrate normally out of r4. (E,F) While floor plate cilia (white arrow) are present and uniformly orientated in the Movl hindbrain (E), they are absent in the MZovl hindbrain (F). Prominent longitudinal axon tracts appear unaffected in MZovl embryos. (G,H) Tag1 in situs to label branchiomotor and sensory neurons. FBM neurons (white arrowheads) migrate out of r4 in both Movl and MZovl embryos, although a small number of neurons (black arrowhead) consistently remained in r4 in MZovl embryos. Asterisks indicate the location of sensory ganglia, which develop normally in both genotypes.

Expression Data

Genes:	cntn2 GFP
Antibody:	Ab1-tuba
Fish:	WT ift88^tz288/tz288 vangl2^tc240/tc240 ift88^tz288/+ rw0Tg dzip1^{ts294e/ts294e}; rw0Tg
Anatomical Terms:	floor plate motile cilium rhombomere 4 branchiomotor neuron rhombomere 6 branchiomotor neuron rhombomere 7 branchiomotor neuron
Stage Range:	26+ somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ift88^tz288/tz288 vangl2^tc240/tc240 ift88^tz288/+ dzip1^{ts294e/ts294e}; rw0Tg
Observed In:	branchiomotor neuron floor plate floor plate motile cilium hindbrain hindbrain motor neuron migration rhombomere 4 branchiomotor neuron rhombomere 6 branchiomotor neuron rhombomere 7 branchiomotor neuron
Stage Range:	26+ somites to Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 382(2), Sittaramane, V., Pan, X., Glasco, D.M., Huang, P., Gurung, S., Bock, A., Li, S., Wang, H., Kawakami, K., Matise, M.P., and Chandrasekhar, A., The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function, 400-412, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.