FIGURE

Fig. 4

ID

ZDB-FIG-131031-25

Publication

Özhan et al., 2013 - Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains

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Fig. 4

lypd6 Acts in Wnt-Receiving Cells at the Level of the Wnt Receptor Complex

(A) pBAR luciferase reporter activity in mouse L cells treated with CM produced from control or Wnt3a-producing L cells that were transfected with lypd6 or control siRNA. Error bars, SEM.

(B) pBAR luciferase reporter activity in Wnt3a CM treated L cells transfected with lypd6 siRNA. Error bars, SEM.

(C) lypd6 enhances Wnt/β-catenin signaling upstream of the β-catenin degradation complex. Coinjection of lypd6 RNA rescues the reduction in reporter activity in 7xTcf:mCherry embryos caused by wnt8 MOs (5 ng each, 17/22), but not by RNAs of axin1 (50 pg, 19/19), gsk3β (150 pg, 17/20), or dominant negative tcf3, tcf3ΔC (125 pg, 23/24) at the 85% epiboly stage.

See also Figure S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 26(4), Özhan, G., Sezgin, E., Wehner, D., Pfister, A.S., Kühl, S.J., Kagermeier-Schenk, B., Kühl, M., Schwille, P., and Weidinger, G., Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains, 331-345, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell