FIGURE

Fig. 5

ID

ZDB-FIG-131017-12

Publication

MacDonald et al., 2013 - The ascl1a and dlx genes have a regulatory role in the development of GABAergic interneurons in the zebrafish diencephalon

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Fig. 5

Exogenous dlx2a and dlx5a mRNA expression in ascl1a and dlx morphants rescues gad1b expression in the diencephalon (prethalamus and hypothalamus). Average number of embryos per injected treatment with normal (blue), and reduced (red) diencephalic gad1b expression. A clear difference in expression is observed between the embryos injected with the control MO+Tol2 mRNA (left insert) and the embryos injected with the ascl1aMO+Tol2 RNA (right insert). The latter is thereafter treated as the baseline for comparison with ascl1aMO+dlx2a (middle insert). Furthermore, exogenous expression of dlx5a, and particularly of dlx2a mRNA, is able to significantly decrease the proportion of embryos with reduced gad1b expression in the diencephalon of dlx1a/2a morphants. Data from 3 experimental replicates, with total individuals per treatment shown within graph ranging from n=144–173, p<0.05.

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Acknowledgments

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Reprinted from Developmental Biology, 381(1), MacDonald, R.B., Pollack, J.N., Debiais-Thibaud, M., Heude, E., Talbot, J.C., and Ekker, M., The ascl1a and dlx genes have a regulatory role in the development of GABAergic interneurons in the zebrafish diencephalon, 276-85, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.