FIGURE

Fig. 2

ID

ZDB-FIG-131007-2

Publication

Andersson et al., 2012 - Adenosine Signaling Promotes Regeneration of Pancreatic beta Cells In Vivo

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Fig. 2

The Hit Compounds Increase Regeneration, Not Survival, of β Cells

(A) Schematic diagram for cell labeling and assessment of β cell survival/regeneration. To examine β cell survival, we made use of the photoconvertible property of the fluorescent protein Kaede. At 3 dpf, before ablating the β cells with MTZ from 3-4 dpf, we converted Tg(ins:Kaede)-expressing β cells from green to red by exposing them to UV light. After 2 days of regeneration (6 dpf), the surviving β cells are red and green (yellow overlap), whereas the newly formed β cells are green only.

(B and C) Confocal images of DMSO- and NECA-treated larvae with Tg(ins:Kaede)-expressing β cells at 6 dpf. Note that there is one β cell that survived the ablation in this particular DMSO-treated larva (arrow in B), whereas there are no β cells that survived the ablation in this NECA-treated larva (C).

(D) Quantification of β cell regeneration (green bars) and β cell survival (yellow bars) per larva at 6 dpf, following treatment with DMSO, NECA, Cilostamide, or A-134974 from 4-6 dpf. p < 0.0001; n = 10 larvae for each group. Error bars represent SEM. See also Figure S2.

Expression Data

Gene:	Kaede
Fish:	s892Tg; s949Tg
Conditions:	chemical treatment: pharmaceutical chemical treatment: drug
Anatomical Term:	pancreatic B cell
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s892Tg; s949Tg
Conditions:	chemical treatment: pharmaceutical chemical treatment: pharmaceutical chemical treatment: pharmaceutical chemical treatment: drug
Observed In:	G protein-coupled adenosine receptor activity pancreas pancreas tissue regeneration pancreatic B cell
Stage:	Day 6

Phenotype Detail

Acknowledgments

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Reprinted from Cell Metabolism, 15(6), Andersson, O., Adams, B.A., Yoo, D., Ellis, G.C., Gut, P., Anderson, R.M., German, M.S., and Stainier, D.Y., Adenosine Signaling Promotes Regeneration of Pancreatic beta Cells In Vivo, 885-894, Copyright (2012) with permission from Elsevier. Full text @ Cell Metab.