FIGURE

Fig. 3

ID

ZDB-FIG-130910-39

Publication

Yao et al., 2013 - Pbx and Prdm1a transcription factors differentially regulate subsets of the fast skeletal muscle program in zebrafish

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Fig. 3

RNA in situ expression of mylpfa (green) and smyhc1 (red) in (A) control, (B) pbx2/4-MO, (C) prdm1a-/-, and (D) pbx2/4-MO;prdm1a-/- embryos. Embryos are 14 somites stage, shown in dorsal view, anterior towards the left. Somite numbers 2–7 are shown for each embryo. Yellow lines indicate where optical sections are taken through the x and y planes (lower and right panels, respectively, each image). Dorsal–ventral (D–V) axes (z axis in xy view) are indicated. Arrowheads point to lateral domain of mylpfa expression, absent or reduced in pbx2/4-MO and pbx2/4-MO;prdm1a-/- (asterisk) embryos. smyhc1-overlapping mylpfa expression in adaxial cells is present in pbx2/4-MO;prdm1a-/- embryos. Scale bar: 50 μm.

Expression Data

Genes:	mylpfa smyhc1
Fish:	WT prdm1a^m805/m805 WT + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4 prdm1a^m805/m805 + MO1-pbx2 + MO2-pbx2 + MO2-pbx4 + MO3-pbx4
Knockdown Reagents:	MO1-pbx2 MO2-pbx2 MO2-pbx4 MO3-pbx4
Anatomical Terms:	adaxial cell slow muscle cell somite
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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