FIGURE

Fig. S4

ID

ZDB-FIG-130907-12

Publication

Alunni et al., 2013 - Notch3 signaling gates cell cycle entry and limits neural stem cell amplification in the adult pallium

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

At the population level, RG remain self-renewing and multipotent upon Notch blockade. (A) Experimental design to test for self-renewal. (B) Cross-section of the adult pallial ventricular zone in a gfap:gfp transgenic brain, triple labeled for GFP (RG, green), CldU (magenta) and MCM5 (blue); white arrows indicate RG cells in proliferation after 48 hours of Notch blockade and still in proliferation at the end of treatment. (C) Experimental design to test for bipotency. (D) Cross-section of the adult pallial ventricular zone in a gfap:gfp transgenic brain, triple labeled for GFP (green), CldU (magenta) and the neuronal marker HuC/D (blue). Some RG cells in proliferation 2 days after the onset of Notch blockade (GFAP/CldU-positive) generated neurons (yellow arrows) and nondividing RG cells (white arrow). Scale bar: 10 μm. Confocal projection images from four optical planes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development