pax3b morphants are metabolically normal but exhibit reduced swallowing capability. All images are lateral view wholemounts with anterior towards the left and dorsal towards the top. (A) Somite-derived hypaxial muscles of 120 hpf Tg(actc1b:egfp) larvae showing PFM (green dots and arrowheads), PHM (white dots and arrowheads) and SHM (yellow dots and arrowheads) muscle reduction and disorganization in pax3b and double morphants. e, eye; asterisk, cranial muscles. (B) Dose dependence of Tg(actc1b:egfp) hypaxial muscle defect in pax3b morphants. (C,D) Migration tracks over 5 minutes of six control (C) and pax3b MO 120 hpf larvae (D). (E,F) Distance swum (E, P=0.87) and time active (F, P=0.73) in a 5-minute period were not significantly different between control and pax3b morphants (unpaired t-tests). Data are mean+s.e.m. (G) qRT-PCR of metabolic marker mRNAs showed no significant difference (pairwise t-tests) between control and pax3b morphants at 120 hpf. Values from four biological replicates (rows 1-4, 10 larvae/sample normalized to 18S rRNA). (H) Fluorescent microspheres fed to 120 hpf larvae were swallowed and detected in the GI tract (arrows). (I) Number of swallowed microspheres in controls and pax morphants. Groups A, B and C differ significantly (P<0.0001). Data are mean+s.e.m. (J,K) Colour-coded regions of GI tract (J) in which microspheres were distributed in control and morphants (K).
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