Fig. 2
- ID
- ZDB-FIG-130722-12
- Publication
- Feng et al., 2012 - The Stress-Response Gene redd1 Regulates Dorsoventral Patterning by Antagonizing Wnt/β-catenin Activity in Zebrafish
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Zebrafish redd1 is a stress-response gene. A) Effect of hypoxia. 6, 24, 36, and 48 hpf old embryos were subjected to 24 h hypoxia treatment (10% of ambient O2 levels). Total RNA was isolated at the indicated developmental stages. The levels of redd1 mRNA were measured by qRT-PCR and normalized by the β-actin mRNA levels. In this and all subsequent figures, the mRNA levels are expressed as a relative value of the control group. Values are means ± S.E. (n = 3). * P<0.05, ** P<0.01, and *** P<0.001. B) Effect of heat shock. Embryos were subjected to 1 h heat shock (37°C) treatment in every 12 h and sampled at 36 hpf and 60 hpf. The levels of redd1 mRNA were measured as described above. C) Effect of food deprivation. Total RNA was isolated from juvenile fish with constant feeding (Feeding) or fasting (Fasting) at indicated time points. The levels of redd1 mRNA were measured and presented as described above. |
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| Stage Range: | Prim-15 to Days 21-29 |