FIGURE

Fig. 3

ID

ZDB-FIG-130618-18

Publication

Villefranc et al., 2013 - A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development

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Fig. 3

vegfc^um18 partially rescues loss of dll4. (A-F) Two-photon micrographs of trunk blood vessels in fixed Tg(fli1a:egfp)^y1 zebrafish embryos at 48 hpf immunostained for Fli1b. Anterior is to the left, dorsal is up. Yellow arrowheads indicate endothelial nuclei. Red arrows indicate ectopic vessel branches. Wild-type (A,B), vegfc^um18/+ (C,D) and vegfc^um18/um18 (E,F) embryos were injected with 15 ng control MO (A,C,E) or 15 ng Dll4 MO (B,D,F). (G,H) Quantification of (G) nuclei and (H) ectopic branches across three ISVs in MO-injected embryos of the indicated genotype. Values are the average of three experiments. ^*P<0.05; N.S., not significant; error bars indicate s.e.m. DA, dorsal aorta; PCV, posterior cardinal vein. Scale bar: 50 μm.

Expression Data

Gene:	fli1rs
Antibody:	Ab1-fli1b
Fish:	WT WT + MO2-dll4 vegfc^um18/+; y1Tg vegfc^um18/um18; y1Tg/y1Tg vegfc^um18/+; y1Tg + MO2-dll4 vegfc^um18/um18; y1Tg/y1Tg + MO2-dll4
Knockdown Reagent:	MO2-dll4
Anatomical Terms:	dorsal aorta intersegmental vessel posterior cardinal vein
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-dll4 vegfc^um18/+; y1Tg vegfc^um18/um18; y1Tg/y1Tg vegfc^um18/+; y1Tg + MO2-dll4 vegfc^um18/um18; y1Tg/y1Tg + MO2-dll4
Knockdown Reagent:	MO2-dll4
Observed In:	angiogenesis endothelial cell intersegmental vessel
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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