FIGURE

Fig. 3

ID

ZDB-FIG-130509-14

Publication

Tao et al., 2013 - Def functions as a cell autonomous factor in organogenesis of digestive organs in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

The def transgene in Tg-I rescues the liver but not intestine and exocrine pancreas development in def^hi429.

(A, B) WISH was performed to analyze the digestive organs in embryos at 4dpf produced by Tg-I^+/ def^hi429/+ × def^hi429/+ using the liver marker fabp10a, intestinal marker fabp2 and exocrine pancreas marker trypsin (A). Embryos from (A) were then individually genotyped and matched to their phenotype. Number of embryos identified in each category was as shown in (B). (C) Progenies from Tg-I^+/ def^hi429/+ × def^hi429/+ at 3.5dpf were also examined simultaneously by Fast Red-labeled fabp10a and trypsin probes BCIP/NBT-labeled fabp2 and insulin probes. Number of embryos in each category was obtained as shown after genotyping a total of 51 embryos from the cross. en: endocrine pancreas, ex: exocrine pancreas, in: intestine, lv: liver.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	utp25^{hi429Tg/hi429Tg} utp25^{hi429Tg/hi429Tg}; zju21Tg/+
Observed In:	digestive tract development exocrine pancreas development liver development
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One