FIGURE

Fig. 5

ID

ZDB-FIG-130423-41

Publication

Tay et al., 2013 - Lethal giant larvae 2 regulates development of the ciliated organ Kupffer's vesicle

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Fig. 5

Genetic interaction between Lgl2 and Rab11a regulates KV lumen formation uncoupled from ciliogenesis. (A,B) Images of GFP-labeled KV cells were used to determine KV lumen volumes (pseudocolored yellow) in live Tg(sox17:GFP) embryos (A) and acetylated tubulin antibodies were used to stain KV cilia (B) in MO control embryos and embryos injected with sub-optimal Rab11a MO^low, Lgl2 MO^low or co-injected with Rab11a MO^low + Lgl2 MO^low. The dashed circle outlines the approximate boundary of KV lumen. Scale bars: 20 μm. (C) Average volume of KV lumen at the 8-somite stage. (D,E) Analysis of KV cilia number (D) and length (E) showed that KV cilia were not significantly affected in Rab11a MO^low + Lgl2 MO^low embryos. Error bars indicate s.d. n, number of embryos analyzed. ^*P<0.05.

Expression Data

Gene:	GFP
Antibody:	Ab1-tuba
Fish:	s870Tg s870Tg + MO1-llgl2 s870Tg + MO1-rab11a s870Tg + MO1-llgl2 + MO1-rab11a
Knockdown Reagents:	MO1-llgl2 MO1-rab11a
Anatomical Terms:	Kupffer's vesicle Kupffer's vesicle motile cilium
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s870Tg + MO1-llgl2 s870Tg + MO1-rab11a s870Tg + MO1-llgl2 + MO1-rab11a
Knockdown Reagents:	MO1-llgl2 MO1-rab11a
Observed In:	Kupffer's vesicle Kupffer's vesicle motile cilium Kupffer's vesicle motile cilium assembly Kupffer's vesicle development
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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