FIGURE

Fig. 1

ID

ZDB-FIG-130423-21

Publication

Liu et al., 2013 - Fibroblast growth factor receptor 2c signaling is required for intestinal cell differentiation in zebrafish

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Fig. 1

Effects on cell differentiation after inhibition of Fgf signaling.
Five dpf embryos were stained with WGA after incubation in (A–C) DMSO, (D–F) SU5402 (2.5 μM), and (G–I) SU5402 (3.4 μM) at 3 dpf. (J–L) Three dpf Tg(hsp70l:dnfgfr1-EGFP) embryos were heat treated, then stained with WGA at 5 dpf. White arrow indicated the goblet cell (gc). DAPI nuclear counter stain showed the tissue structure. (M) Heat-treated WT embryos were used as controls. (N) Heat shocked transgenic embryos were double labeled with WGA and 2F11 antibody at 5 dpf. (M′ ,N′) The magnified image shows enteroendocrine and (M′ ′,N′ ′) goblet cells. (O) The bar charts show the percentage of 2F11 or WGA positive cells. All images were lateral view with anterior at left and posterior at right. Error bars indicate SD. Scale bars = 50 μm.

Expression Data

Antibody:	Ab-2F11
Fish:	WT pd1Tg
Condition:	heat shock
Anatomical Term:	enteroendocrine cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT pd1Tg
Conditions:	chemical treatment: SU5402 heat shock
Observed In:	enteroendocrine cell goblet cell intestine
Stage:	Day 5

Phenotype Detail

Acknowledgments

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