FIGURE

Fig. S10

ID

ZDB-FIG-130423-20

Publication

Stoll et al., 2013 - HOXC9 Regulates Formation of Parachordal Lymphangioplasts and the Thoracic Duct in Zebrafish via Stabilin 2

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Fig. S10

Expression silencing of Stab1 and Stab2 shows no additive effect on parachordal lymphangioplast (PL) assembly.
(A,D) Overall morphology of 48 hpf (A) and 72 hpf (D) zebrafish embryos after injection of control morpholino. Red box shows region displayed in (B,C,E,F). (B,E) Normal formation of the PLs (arrows) in 48 hpf (B) and 72 hpf (E) tg(fli1:EGFP) zebrafish embryos after injection of 4 ng control morpholino. (C,F) Double silencing of Stab1 and Stab2 expression using 4 ng of each splice-blocking morpholino for Stab1 and Stab2, respectively, disrupted formation of the PLs (asterisks) in 48 hpf (C) and 72 hpf (F) tg(fli1:EGFP) zebrafish embryos. (G,H) Quantification of 48 hpf (G) and 72 hpf (H) tg(fli1:EGFP) zebrafish embryos showing a disturbed PL formation. Embryos were divided in three groups depending on the PL appearance being completely absent, partially formed or completely present. Black scale bar: 500 μm. White scale bar: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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