FIGURE

Fig. 3

ID

ZDB-FIG-130411-30

Publication

Veerkamp et al., 2013 - Unilateral dampening of bmp activity by nodal generates cardiac left-right asymmetry

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Fig. 3

Nodal and Bmp Signaling Affect Cardiac Progenitor Cell Motility and Laterality(A) Quantifications of cell motilities based on time-lapse analyses of endothelial and myocardial reporter lines between 19 and 23 hpf. In WT, endocardial (green) and myocardial (red) progenitor cell motility rates are significantly different between the left and right (mean with SD based on quantifications of at least n = 22 endocardial and n = 30 myocardial cells per genotype; p < 0.025), whereas no significant L/R differences are observed in spaw morphants.(B) Average speed measurements for spaw morphants or Bmp2b-overexpressing endocardial (green) and myocardial (red) progenitor cells reveal highly significant differences compared with WT (mean with SD based on quantifications of at least n = 16 endocardial cells and n = 16 myocardial cells per genotype; p < 0.0005).(C) Myocardial (red) and endocardial (green) progenitor cell tracks based on live imaging. Bearing angles summarize all endocardial and myocardial cell tracks. ns, not significant.See also Movies S3 and S4.

Expression Data

Genes:	EGFP mRFP
Fish:	md3Tg; y1Tg fr13Tg; md3Tg; y1Tg md3Tg; y1Tg + MO1-spaw
Condition:	heat shock
Knockdown Reagent:	MO1-spaw
Anatomical Terms:	heart rudiment endocardial precursor heart rudiment myocardial precursor
Stage Range:	20-25 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fr13Tg; md3Tg; y1Tg md3Tg; y1Tg + MO1-spaw
Condition:	heat shock
Knockdown Reagent:	MO1-spaw
Observed In:	cardioblast migration cell migration involved in heart jogging heart jogging heart rudiment endocardial precursor heart rudiment myocardial precursor
Stage Range:	20-25 somites to 26+ somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 24(6), Veerkamp, J., Rudolph, F., Cseresnyes, Z., Priller, F., Otten, C., Renz, M., Schaefer, L., and Abdelilah-Seyfried, S., Unilateral dampening of bmp activity by nodal generates cardiac left-right asymmetry, 660-667, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell