FIGURE

Fig. 5

ID

ZDB-FIG-130404-25

Publication

Doganli et al., 2012 - The alpha2Na+/K+-ATPase is critical for skeletal and heart muscle function in zebrafish

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Fig. 5

Atp1a2 expression colocalizes with GFAP, and Atp1a2 knockdown causes abnormal GFAP distribution. (A) Atp1a2 transcript colocalizes with the astrocytic marker GFAP in the embryonic zebrafish brain (region indicated by a red rectangle and an asterisk in the drawing). Transgenic embryos expressing GFP driven by the Gfap promoter were double stained using in situ hybridization for Atp1a2 (red fluorescence) in combination with anti-GFP immunostaining (green fluorescence). Arrows indicate Atp1a2/GFAP co-expressing cells, seen in yellow. (B) Distribution of Gfap transcripts, assessed by in situ hybridization, in std-MO- and α₂-MO-injected embryos is distorted in α₂-MO-injected embryos. Similarly, distribution of GFAP protein in Tg(Gfap:GFP) embryos is distorted by Atp1a2 knockdown. HB, hindbrain; MB, midbrain; SC, spinal cord; VZ, forebrain ventricular zone. Scale bars: 5μm.

Expression Data

Genes:	atp1a2a gfap
Fish:	mi2001Tg mi2001Tg + MO1-atp1a2a
Knockdown Reagent:	MO1-atp1a2a
Anatomical Terms:	astrocyte central nervous system hindbrain midbrain posterior region spinal cord dorsal region ventricular zone
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.