FIGURE

Fig. 7

ID

ZDB-FIG-130404-11

Publication

Law et al., 2013 - Maternal pak4 Expression is Required for Primitive Myelopoiesis in Zebrafish

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Fig. 7

Disruption of actin microfilaments leads to loss of scl expression in the RBI. Dechorinated embryos at tailbud stage were exposed to cytochalasin B (4 μg/ml) or latrunculin B (0.25 μg/ml) and fixed at 5–6 somite stage for whole-mount in situ hybridization using the ,scl gene probe. 30–40% Mortality was observed in all drug exposures due to rupture of the enveloping layer at variable times. Dead embryos were not analyzed further. Flat mounts of representative embryos from cytochalasin B exposure (A–C; normal, posterior expression only, no expression) and DMSO control (D) were prepared to show the anterior and posterior expression of scl. (E) Data were summarized from four independent experiments which show that the majority of embryos specifically lost the anterior scl expression after drug treatments. Posterior-specific loss of expression was never observed.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 130(2-3), Law, S.H., and Sargent, T.D., Maternal pak4 Expression is Required for Primitive Myelopoiesis in Zebrafish, 181-194, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.