FIGURE

Fig. 5

ID

ZDB-FIG-130326-55

Publication

Fraser et al., 2013 - Regeneration of cone photoreceptors when cell ablation is primarily restricted to a particular cone subtype

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Fig. 5

Morphological analysis of photoreceptors indicates that metronidazole does not damage neighbouring cells.

UV cones expressing nitroreductase (NTR) were ablated after the addition of prodrug metronidazole (MTZ) (A–D), while nfsB-mCherry persisted in transgenic fish treated with DMSO vehicle control (E–H). 24 hours after ablation, UV cones lacking NTR-expression appeared morphologically normal (C) based on their GFP expression compared to controls (G). Red-green double cone pairs were detected with the antibody zpr1. Zpr1 labelling was consistent following MTZ ablation (B) showing normal cell morphology compared to the controls (F). Scale bar = 25 µm. A magnified view of remaining UV cones (I) with normal morphology comparable to controls is presented from a separate sample (J, Scale bar = 5 µm).

Expression Data

Genes:	EGFP mCherry
Antibody:	zpr-1
Fish:	c264Tg; kj9Tg; ua3016Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Terms:	green sensitive photoreceptor cell red sensitive photoreceptor cell UV sensitive photoreceptor cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	c264Tg; kj9Tg; ua3016Tg
Condition:	chemical treatment: pharmaceutical
Observed In:	retina UV sensitive photoreceptor cell
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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