FIGURE

Fig. 1

ID

ZDB-FIG-130313-19

Publication

Jing et al., 2013 - Phospholipase c gamma-1 is required for granulocyte maturation in zebrafish

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Fig. 1

Mutants of ldd239 display granulopoiesis and angiogenesis defects. ((A)–(D)) WISH analysis of mpx expression at 18 hpf and 22 hpf. In ldd239 mutants, mpx has an obviously decreased expression in the ICM compared with wild-type siblings at 18 hpf ((A) and (B), black arrows) and 22 hpf ((C) and (D), black arrows). Black arrows indicate the mpx+ cells in the ICM. White arrows represent the mpx+ cells in the A-LPM which have no significant difference between siblings and mutants ((C) and (D), white arrows). ((E)–(J)) WISH assays of fli1 and flk1 expression at 18 hpf and 22 hpf. Expression of fli1 has no difference between siblings and mutants at 18 hpf ((E) and (F)). Expression of fli1 and flk1 at 22 hpf show deficient angiogenesis and defects are better shown in higher magnifications ((G)–(J), black arrows). ((K)–(N)) Expression of lyz ((K) and (L)) and l-plastin ((M) and (N)) are normal in the A-LPM of mutants at 22 hpf. ((O)–(T)) Hematopoietic progenitor marker, scl ((O) and (P)), erythrocyte progenitors marker, gata1 ((Q) and (R)), and mature erythrocyte marker, hemoglobin hbae1, expression of all markers have no change in mutants at 22 hpf.

Expression Data

Genes:	gata1a hbae1.1 lcp1 lyz mpx tal1
Fish:	WT plcg1^rj100/rj100 (TU)
Anatomical Terms:	erythroblast hematopoietic multipotent progenitor cell intermediate cell mass of mesoderm lateral plate mesoderm nucleate erythrocyte
Stage Range:	14-19 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	plcg1^rj100/rj100 (TU)
Observed In:	angiogenesis granulocyte differentiation myeloid leukocyte differentiation
Stage Range:	14-19 somites to 26+ somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 374(1), Jing, C.B., Chen, Y., Dong, M., Peng, X.L., Jia, X.E., Gao, L., Ma, K., Deng, M., Liu, T.X., Zon, L.I., Zhu, J., Zhou, Y., and Zhou, Y., Phospholipase c gamma-1 is required for granulocyte maturation in zebrafish, 24-31, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.