Fig. 2

Wnt8a overexpression does not induce the posterior migration of hepatoblasts.

The lineage of endodermal cells at the 2^nd, 3^rd, or 4^th somite level was traced. (A–C) Lineage tracing of endodermal cells at the 2^nd somite level. The distribution of Kik^Red+ cells in control is similar to that in Wnt8a-overexpressing embryos. Cartoons incorporating all lineage-tracing data (C). (D–H) Lineage tracing of endodermal cells at the 3^rd somite level. Most Kik^Red+ cells expressed Prox1 (green) in Wnt8a-overexpressing embryos (G, open arrowheads), whereas a small number of Kik^Red+ cells did in controls (E). Higher magnification images of the square regions in D and F are shown in E and G, respectively, without Pdx1 and Kik^green expression. (I–M) Lineage tracing of endodermal cells at the 4^th somite. Many Kik^Red+ cells expressed Prox1 (green) in Wnt8a-overexpressing embryos (L, open arrowheads), whereas none of the cells did in controls (J). Higher magnification images of the square regions in I and K are shown in J and L, respectively, without Pdx1 and Kik^green expression. Green and blue lines outline Prox1⁺ and Pdx1⁺ domains, respectively, and n indicates the number of embryos (C,H,M). Brackets delineate the endodermal region in which ectopic hepatoblasts are present (B–B3,F,K). Dotted lines outline the dorsal pancreas (DP). Ventral views, anterior up. Scale bars, 20µm.