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Fig. 1

ID

ZDB-FIG-130225-2

Publication

Zhang et al., 2012 - AgRP and POMC neurons are hypophysiotropic and coordinately regulate multiple endocrine axes in a larval teleost

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Fig. 1

agrp Is Required for Normal Somatic Growth in Larval Zebrafish

(A–D) Representative fish at 3 dpf following injection at day 0 with morpholino oligonucleotides designed to inhibit expression of each of the zebrafish agouti proteins (A). Body lengths (jaw to tail fin) of injected fish were measured using a micrometer at 5 dpf, showing a dose-responsive inhibition of growth following morpholino blockade of agrp expression (B). Body lengths corresponding to fish injected at day 0 were measured at 14 dpf, showing partial restoration of length (C). Body lengths at 5 dpf following injection of zygotes with 5 ng morpholino oligonucleotide are indicated, plus 30 pg capped agrp RNA, where indicated, showing partial rescue of growth (D). Bars indicate mean ± SEM. n = 30 (B), 10 (C), and 18–23 (D). Statistical significance was tested by one-way ANOVA followed by Tukey′s post test (p < 0.05; p < 0.01; p < 0.001). See also Figure S1

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-agrp
Knockdown Reagent:	MO1-agrp
Observed In:	developmental growth somite whole organism
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Cell Metabolism, 15(2), Zhang, C., Forlano, P.M., and Cone, R.D., AgRP and POMC neurons are hypophysiotropic and coordinately regulate multiple endocrine axes in a larval teleost, 256-264, Copyright (2012) with permission from Elsevier. Full text @ Cell Metab.