FIGURE

Fig. S4

ID

ZDB-FIG-130213-13

Publication

Lalwani et al., 2012 - Reverse Genetics Screen in Zebrafish Identifies a Role of miR-142a-3p in Vascular Development and Integrity

Other Figures

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Fig. S4

Microinjection of lmo2 1&2 morpholino (MO) in Tg(fli1:EGFP, gata1a: dsRed) zebrafish embryos. A-H - Morpholino (MO) mediated Lmo2 knockdown induces blood cells and blood vessel defects in 2 dpf Tg(fli1:EGFP, gata1a: dsRed) zebrafish embryos. A?D - non-injected control (NIC) and E ? H 1.75 ng lmo2 1&2 MO injected zebrafish embryos. A?C, E ? G Apotome microscope images (GFP/RFP merged) of lmo2 1&2 MO injected Tg(fli1:EGFP, gata1a: dsRed) embryos at 2 dpf (Lateral View). A?C - non-injected control embryos and E ? G ?lmo2 1&2 MO injected embryos. D, H - Embryos stained with o-dianisidine. The embryos were imaged at 10� magnification (A?C, E?G) and 2.5� magnification (D, H). Arrowheads indicate the site of defects.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sd2Tg; y1Tg + MO1-lmo2 + MO2-lmo2
Knockdown Reagents:	MO1-lmo2 MO2-lmo2
Observed In:	blood vessel nucleate erythrocyte trunk intersegmental vessel
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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