Fig. 1

Nuclear access is required for Wdr68 function in craniofacial development.

A-H) Alcian blue stained animals. A, C, E, G) Ventral view with the Meckel′s (M), palatoquadrate (PQ) and ceratohyal (CH) labeled in red. B, D, F, H) Lateral view. A, B) Wildtype control-MO (ctrl) injected animals. C, D) Severe craniofacial phenotype of wdr68-MO injected animals co-injected with GFP mRNA (MO+GFP) that fails to rescue the craniofacial defects. E, F) Rescue of the craniofacial defects of wdr68-MO animals co-injected with GFP-Wdr68 mRNA (MO+GW). G, H) Severe craniofacial defects of wdr68-MO animals co-injected with GFPNESWdr68 mRNA (MO+GNESW) that fails to rescue the craniofacial defects. I) Graph summarizing the results of three injection trials. In aggregate, n = 50/50 (100%) for control animals, n = 47/257 (18%) for MO+GFP animals, n = 212/350 (61%) for MO+GW animals, and n = 71/385 (18%) for MO+GNESW animals.